A simple, accurate and precise dual wavelength spectrophotometric method was developed for simultaneous determination of amiloride hydrochloride and furosemide in combined pharmaceutical dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of amiloride hydrochloride were 281 nm and 271 nm, whereas, the wavelengths selected for determination of furosemide were 280 nm and 290 nm. Methanol was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 2-10 μg/ml for amiloride hydrochloride and 16-80 μg/ml for furosemide. Accuracy of method was found within limits. The precision (intra‐day, inter‐day and repeatability) of method was found within limits. The methods were validated according to ICH guidelines for evaluation of accuracy, LOD and LOQ, precision, repeatability, reproducibility etc. The proposed method was successfully applied to determination of these drugs in commercial tablets.
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